• Western blot analysis of extracts of various cell lines, using CORO1A antibody (STJ111647) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using CORO1A Rabbit polyclonal antibody (STJ111647) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat spleen using CORO1A rabbit polyclonal antibody (STJ111647) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using CORO1A Rabbit polyclonal antibody (STJ111647) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human thyroid cancer using CORO1A rabbit polyclonal antibody (STJ111647) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using CORO1A Rabbit polyclonal antibody (STJ111647) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using CORO1A rabbit polyclonal antibody (STJ111647) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using CORO1A Rabbit polyclonal antibody (STJ111647) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse liver using CORO1A rabbit polyclonal antibody (STJ111647) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using CORO1A Rabbit polyclonal antibody (STJ111647) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using CORO1A rabbit polyclonal antibody (STJ111647) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CORO1A antibody (STJ111647) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CORO1A antibody (362-461) (STJ111647)

SKU:
STJ111647

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CORO1A (362-461) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CORO1A
Gene ID: 11151
Uniprot ID: COR1A_HUMAN
Immunogen Region: 362-461
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 362-461 of human CORO1A (NP_001180262.1).
Immunogen Sequence: DLYPPTAGPDPALTAEEWLG GRDAGPLLISLKDGYVPPKS RELRVNRGLDTGRRRAAPEA SGTPSSDAVSRLEEEMRKLQ ATVQELQKRLDRLEETVQAK
Tissue Specificity Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.
Post Translational Modifications phosphorylation at Thr-412 by PKC strongly down-regulates the association with actin. Polyubiquitinated by RNF128 with 'Lys-48'-linked chains, leading to proteasomal degradation.
Function May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.
Protein Name Coronin-1a
Coronin-Like Protein A
Clipin-A
Coronin-Like Protein P57
Tryptophan Aspartate-Containing Coat Protein
Taco
Database Links Reactome: R-HSA-9636383
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Cytoplasmic Vesicle
Phagosome Membrane
In Non-Infected Macrophages
Associated With The Cortical Microtubule Network
In Mycobacteria-Infected Macrophages
Becomes Progressively Relocalized And Retained Around The Mycobacterial Phagosomes
Retention On The Phagosomal Membrane Is Strictly Dependent On Mycobacterial Viability And Not Due To Impaired Acidification
Alternative Antibody Names Anti-Coronin-1a antibody
Anti-Coronin-Like Protein A antibody
Anti-Clipin-A antibody
Anti-Coronin-Like Protein P57 antibody
Anti-Tryptophan Aspartate-Containing Coat Protein antibody
Anti-Taco antibody
Anti-CORO1A antibody
Anti-CORO1 antibody

Information sourced from Uniprot.org