Anti-COP1/RFWD2 antibody (582-731 aa) [SRM5264] (STJ11107166)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal COP1/RFWD2 (582-731 aa) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5264 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | COP1 |
| Gene ID | 64326 |
| Uniprot ID | COP1_HUMAN |
| Immunogen Region | 582-731 aa |
| Immunogen Sequence | YYDLRNTKQPIMVFKGHRKA VSYAKFVSGEEIVSASTDSQ LKLWNVGKPYCLRSFKGHIN EKNFVGLASNGDYIACGSEN NSLYLYYKGLSKTLLTFKFD TVKSVLDKDRKEDDTNEFVS AVCWRALPDGESNVLIAANS QGTIKVLELV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 582-731 of human COP1/RFWD2 (NP_071902.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart. |
| Post Translational Modifications | Autoubiquitinated. MTA1 destabilizes it by promoting its autoubiquitination. |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable). |
| Protein Name | E3 Ubiquitin-Protein Ligase Cop1Constitutive Photomorphogenesis Protein 1 HomologHcop1Ring Finger And Wd Repeat Domain Protein 2Ring Finger Protein 200Ring-Type E3 Ubiquitin Transferase Rfwd2 |
| Database Links | Reactome: R-HSA-349425Reactome: R-HSA-8951664 |
| Cellular Localisation | Nucleus SpeckleCytoplasmIn The NucleusIt Forms Nuclear Speckles |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Cop1 antibodyAnti-Constitutive Photomorphogenesis Protein 1 Homolog antibodyAnti-Hcop1 antibodyAnti-Ring Finger And Wd Repeat Domain Protein 2 antibodyAnti-Ring Finger Protein 200 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rfwd2 antibodyAnti-COP1 antibodyAnti-RFWD2 antibodyAnti-RNF200 antibody |
Information sourced from Uniprot.org