• Western blot analysis of 293T, using COP1 Rabbit polyclonal antibody (STJ11104711) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-COP 1 antibody (582-731) (STJ11104711)

SKU:
STJ11104711

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 Ubiquitin-Protein Ligase Cop1 (582-731) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COP1
Gene ID: 64326
Uniprot ID: COP1_HUMAN
Immunogen Region: 582-731
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 582-731 of human COP1 (NP_071902.2).
Immunogen Sequence: YYDLRNTKQPIMVFKGHRKA VSYAKFVSGEEIVSASTDSQ LKLWNVGKPYCLRSFKGHIN EKNFVGLASNGDYIACGSEN NSLYLYYKGLSKTLLTFKFD TVKSVLDKDRKEDDTNEFVS AVCWRALPDGESNVLIAANS QGTIKVLELV
Tissue Specificity Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
Post Translational Modifications Autoubiquitinated. MTA1 destabilizes it by promoting its autoubiquitination.
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).
Protein Name E3 Ubiquitin-Protein Ligase Cop1
Constitutive Photomorphogenesis Protein 1 Homolog
Hcop1
Ring Finger And Wd Repeat Domain Protein 2
Ring Finger Protein 200
Ring-Type E3 Ubiquitin Transferase Rfwd2
Database Links Reactome: R-HSA-349425
Reactome: R-HSA-8951664
Cellular Localisation Nucleus Speckle
Cytoplasm
In The Nucleus
It Forms Nuclear Speckles
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Cop1 antibody
Anti-Constitutive Photomorphogenesis Protein 1 Homolog antibody
Anti-Hcop1 antibody
Anti-Ring Finger And Wd Repeat Domain Protein 2 antibody
Anti-Ring Finger Protein 200 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rfwd2 antibody
Anti-COP1 antibody
Anti-RFWD2 antibody
Anti-RNF200 antibody

Information sourced from Uniprot.org

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