• Dot-blot analysis of all sorts of peptides using Collagen IV Rabbit monoclonal antibody antibody (STJ11105279) at 1:1000 dilution.
  • Western blot analysis of HeLa, using Collagen IV Rabbit monoclonal antibody (STJ11105279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Collagen IV antibody (1573-1690) [S5279RM] (STJ11105279)

SKU:
STJ11105279

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Collagen IV (1573-1690) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5279RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000DB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1573-1690
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1573-1690 of human COL4A4 (NP_000083.3).
Immunogen Sequence: AQAVAVHSQDQSIPPCPQTW RSLWIGYSFLMHTGAGDQGG GQALMSPGSCLEDFRAAPFL ECQGRQGTCHFFANKYSFWL TTVKADLQFSSAPAPDTLKE SQAQRQKISRCQVCVKYS
Background This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3 UTR.

Information sourced from Uniprot.org

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