• Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using Cold1 Rabbit polyclonal antibody (STJ11104070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-Cold1 antibody (90-260 aa) (STJ11104070)

SKU:
STJ11104070

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Host: Rabbit
Applications: WB/ELISA
Reactivity: O.sativa
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 90-260 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 90-260 of Oryza sativa Cold1. (Q7X7S8).
Immunogen Sequence: VFVLPYYHCYLLLRNSGVRR ERALLVAALFLLVFLYGFWR MGIHFPMPSPEKGFFTMPQL VSRIGVIGVSVMAVLSGFGA VNLPYSYLSLFIREIDEKDI KTLERQLMQSMETCIAKKKK IVLSKMEMERIQGSEEKLKA RSFLKRIVGTVVRSVQEDQT EQDIKSLDAEV
Background Involved in chilling tolerance. Interacts with the G-protein alpha subunit GPA1 to activate the calcium channel for sensing low temperature and to accelerate G-protein GTPase activity.

Information sourced from Uniprot.org

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