• Western blot analysis of extracts of various cell lines, using COL9A1 Rabbit polyclonal antibody antibody (STJ28646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-COL9A1 antibody (20-270) (STJ28646)

SKU:
STJ28646

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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COL9A1 (20-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL9A1
Gene ID: 1297
Uniprot ID: CO9A1_HUMAN
Immunogen Region: 20-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-270 of human COL9A1 (NP_001842.3).
Immunogen Sequence: WASAAVKRRPRFPVNSNSNG GNELCPKIRIGQDDLPGFDL ISQFQVDKAASRRAIQRVVG SATLQVAYKLGNNVDFRIPT RNLYPSGLPEEYSFLTTFRM TGSTLKKNWNIWQIQDSSGK EQVGIKINGQTQSVVFSYKG LDGSLQTAAFSNLSSLFDSQ WHKIMIGVERSSATLFVDCN RIESLPIKPRGPIDIDGFAV LGKLADNPQVSVPFELQWML IHCDPLRPRRETCHELPAR
Post Translational Modifications Covalently linked to the telopeptides of type II collagen by lysine-derived cross-links. Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function Structural component of hyaline cartilage and vitreous of the eye.
Protein Name Collagen Alpha-1(Ix Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000178
Reactome: R-HSA-419037
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(Ix Chain antibody
Anti-COL9A1 antibody

Information sourced from Uniprot.org

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