Western blot analysis of lysates from 1) MCF7, 2) Hela, 3) 293T cells, (Green) primary antibody was diluted at 1:1000, 4ยฐC over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37ยฐC 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4ยฐC over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37ยฐC 1hour.

Anti-COL3A1 antibody [Q76] (STJ97025)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenSynthetic Peptide of Collagen III
STJ97025
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General Information

Short DescriptionMouse monoclonal anti-Collagen alpha-1III chain for use in IHC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC/IF
HostMouse
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDQ76
IsotypeIgG1
ConjugationUnconjugated
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution RangeIF 1:200
IHC 1:50-300
FormulationLiquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCOL3A1
Gene ID1281
Uniprot IDCO3A1_HUMAN
ImmunogenSynthetic Peptide of Collagen III
SpecificityThe antibody detects endogenous Collagen III protein.

Additional Info

Function Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
Protein Name Collagen Alpha-1(Iii Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-198933
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000170
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-3000480
Reactome: R-HSA-419037
Reactome: R-HSA-8874081
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(Iii Chain antibody
Anti-COL3A1 antibody

Information sourced from Uniprot.org

Citations

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