| Host: |
Rabbit |
| Applications: |
ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
COL12A1 |
| Gene ID: |
1303 |
| Uniprot ID: |
COCA1_HUMAN |
| Immunogen Region: |
24-270 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 24-270 of human COL12A1 (NP_004361.3). |
| Immunogen Sequence: |
EVDPPSDLNFKIIDENTVHM SWAKPVDPIVGYRITVDPTT DGPTKEFTLSASTTETLLSE LVPETEYVVTITSYDEVEES VPVIGQLTIQTGSSTKPVEK KPGKTEIQKCSVSAWTDLVF LVDGSWSVGRNNFKYILDFI AALVSAFDIGEEKTRVGVVQ YSSDTRTEFNLNQYYQRDEL LAAIKKIPYKGGNTMTGDAI DYLVKNTFTESAGARVGFPK VAIIITDGKSQDEVEIPAR |
| Tissue Specificity | Found in collagen I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine, and in cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, kidney and a squamous cell carcinoma cell line. Isoform 1 is also present in the corneal epithelial Bowman's membrane (BM) and the interfibrillar matrix of the corneal stroma, but it is not detected in the limbal BM. |
| Post Translational Modifications | The triple-helical tail is stabilized by disulfide bonds at each end. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. Isoform 1 O-glycosylation.glycosaminoglycan of chondroitin-sulfate type. |
| Function | Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix. |
| Protein Name | Collagen Alpha-1(Xii Chain |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1650814Reactome: R-HSA-2022090Reactome: R-HSA-8948216 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Collagen Alpha-1(Xii Chain antibodyAnti-COL12A1 antibodyAnti-COL12A1L antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
