| Host: |
Rabbit |
| Applications: |
DB/ELISA |
| Reactivity: |
Species independent |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4155RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
DB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Background | The coenzyme NAD is involved in oxidation–reduction reactions critical for glycolysis, fatty acid oxidation, the TCA cycle, and complex I of the mitochondrial respiratory chain and also is a key regulator of autophagy. At least two different mechanisms are involved. First, the NAD+-dependent deacetylase SIRT1 activates autophagy by directly deacetylating ATG proteins. Under starvation conditions, the increased NAD+/NADH ratio activates SIRT1, which results in stimulation of mitophagy. Second, the hydrogen of NADH can be transferred to NADP+ to form NADPH via the energy-linked transhydrogenase. In the fed state, when the NAD+/NADH ratio falls, NADPH inhibits autophagy by scavenging of ROS via the glutathione peroxidase-glutathione reductase system and by preventing the production of ROS at complex 1 of the respiratory chain. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
