Anti-COBRA1 antibody (Full-Length) [F7E4] (STJA0003598)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenRecombinant full length human COBRA1 purification from E. coli.
General Information
| Short Description | Mouse monoclonal anti-COBRA1 (Full-Length) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | F7E4 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | This antibody was protein g purified culture from supernatant. |
| Dilution Range | WB 1:1000 |
| Formulation | 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NELFB |
| Gene ID | 25920 |
| Uniprot ID | NELFB_HUMAN |
| Immunogen | Recombinant full length human COBRA1 purification from E. coli. |
| Immunogen Region | Full-Length |
Additional Info
| Tissue Specificity | Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas. |
| Function | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding. Essential for early embryogenesis.plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes. Plays a key role in establishing the responsiveness of stem cells to developmental cues.facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules. Supports the transcription of genes involved in energy metabolism in cardiomyocytes.facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. |
| Protein Name | Negative Elongation Factor BNelf-BCofactor Of Brca1 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167242Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-75955Reactome: R-HSA-9603505 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Negative Elongation Factor B antibodyAnti-Nelf-B antibodyAnti-Cofactor Of Brca1 antibodyAnti-NELFB antibodyAnti-COBRA1 antibodyAnti-KIAA1182 antibody |
Information sourced from Uniprot.org