• Immunohistochemistry analysis of CNTN1 in paraffin-embedded mouse brain using CNTN1 Rabbit polyclonal antibody (STJ119575) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CNTN1 in paraffin-embedded mouse spinal cord using CNTN1 Rabbit polyclonal antibody (STJ119575) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CNTN1 in paraffin-embedded human placenta using CNTN1 Rabbit polyclonal antibody (STJ119575) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CNTN1 in paraffin-embedded Rat brain using CNTN1 Rabbit polyclonal antibody (STJ119575) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CNTN1 Rabbit polyclonal antibody (STJ119575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CNTN1 antibody (250-350) (STJ119575)

SKU:
STJ119575

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CNTN1 (250-350) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CNTN1
Gene ID: 1272
Uniprot ID: CNTN1_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human CNTN1 (NP_778203.1).
Immunogen Sequence: LECFALGNPVPDIRWRKVLE PMPSTAEISTSGAVLKIFNI QLEDEGIYECEAENIRGKDK HQARIYVQAFPEWVEHINDT EVDIGSDLYWPCVATGKPIP T
Tissue Specificity Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.
Function Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.
Protein Name Contactin-1
Glycoprotein Gp135
Neural Cell Surface Protein F3
Database Links Reactome: R-HSA-2122948
Reactome: R-HSA-2979096
Reactome: R-HSA-373760
Reactome: R-HSA-447043
Cellular Localisation Isoform 1: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Isoform 2: Cell Membrane
Alternative Antibody Names Anti-Contactin-1 antibody
Anti-Glycoprotein Gp135 antibody
Anti-Neural Cell Surface Protein F3 antibody
Anti-CNTN1 antibody

Information sourced from Uniprot.org

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