| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CNP |
| Gene ID: | 1267 |
| Uniprot ID: | CN37_HUMAN |
| Immunogen Region: | 150-421 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 150-421 of human CNPasease (NP_149124.3). |
| Immunogen Sequence: | PKTAWRLDCAQLKEKNQWQL SADDLKKLKPGLEKDFLPLY FGWFLTKKSSETLRKAGQVF LEELGNHKAFKKELRQFVPG DEPREKMDLVTYFGKRPPGV LHCTTKFCDYGKAPGAEEYA QQDVLKKSYSKAFTLTISAL FVTPKTTGARVELSEQQLQL WPSDVDKLSPTDNLPRGSRA HITLGCAADVEAVQTGLDLL EILRQEKGGSRGEEVGELSR GKLYSLGNGRWMLTLAKNM |
| Function | Catalyzes the formation of 2'-nucleotide products from 2',3'-cyclic substrates. May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. |
| Protein Name | 2' -3'-Cyclic-Nucleotide 3'-PhosphodiesteraseCnpCnpase |
| Cellular Localisation | MembraneLipid-AnchorMelanosomeFirmly Bound To Membrane Structures Of Brain White Matter |
| Alternative Antibody Names | Anti-2' -3'-Cyclic-Nucleotide 3'-Phosphodiesterase antibodyAnti-Cnp antibodyAnti-Cnpase antibodyAnti-CNP antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and CNPasease knockout (KO) HeLa cells, using [KO Validated] CNPase Rabbit polyclonal antibody (STJ119982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98767/382818/STJ119982_1__79726.1713151315.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CNPasease knockout (KO) HeLa cells, using [KO Validated] CNPase Rabbit polyclonal antibody (STJ119982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
