Anti-CNOT1 antibody (2137-2376) (STJ117822)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CNOT1 (2137-2376) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CNOT1 |
| Gene ID | 23019 |
| Uniprot ID | CNOT1_HUMAN |
| Immunogen Region | 2137-2376 |
| Immunogen Sequence | PRNMRLPDPFTPNLKVDMLS EINIAPRILTNFTGVMPPQF KKDLDSYLKTRSPVTFLSDL RSNLQVSNEPGNRYNLQLIN ALVLYVGTQAIAHIHNKGST PSMSTITHSAHMDIFQNLAV DLDTEGRYLFLNAIANQLRY PNSHTHYFSCTMLYLFAEAN TEAIQEQITRVLLERLIVNR PHPWGLLITFIELIKNPAFK FWNHEFVHCAPEIEKLFQSV AQCCMGQKQAQQVMEGTGA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 2137-2376 of human CNOT1 (NP_057368.3). |
Additional Info
| Tissue Specificity | Strongly expressed in brain, heart, thymus, spleen, kidney, liver, placenta and lung. Weakly expressed in skeletal muscle and colon. |
| Function | Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity. Plays a role in rapid sperm motility via mediating timely mRNA turnover. |
| Protein Name | Ccr4-Not Transcription Complex Subunit 1Ccr4-Associated Factor 1Negative Regulator Of Transcription Subunit 1 HomologNot1hHnot1 |
| Database Links | Reactome: R-HSA-429947Reactome: R-HSA-6804115Reactome: R-HSA-9820841 |
| Cellular Localisation | CytoplasmP-BodyNucleusNanos2 Promotes Its Localization To P-Body |
| Alternative Antibody Names | Anti-Ccr4-Not Transcription Complex Subunit 1 antibodyAnti-Ccr4-Associated Factor 1 antibodyAnti-Negative Regulator Of Transcription Subunit 1 Homolog antibodyAnti-Not1h antibodyAnti-Hnot1 antibodyAnti-CNOT1 antibodyAnti-CDC39 antibodyAnti-KIAA1007 antibodyAnti-NOT1 antibodyAnti-AD-005 antibody |
Information sourced from Uniprot.org