Anti-CNF1/CNF2 antibody [JC4] (STJ16100793)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
General Information
| Short Description | Mouse monoclonal anti-CNF1/CNF2 for use in FUNC, ELISA and WB in samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FUNC/ELISA/WB |
| Host | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | JC4 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 100 Mu g/mL |
| Formulation | PBS with 0.1% BSA and 0.02% sodium azide |
| Storage Instruction | Store at 2-8°C for up to 1-year, upon receipt. |
Target Information
Additional Info
| Background | The monoclonal antibody JC4 is specific for Cytotoxic necrotizing factor type 1 and the highly related Cytotoxic necrotizing factor type 2 (CNF1 and CNF2) of uropathogenic Escherichia coli. CNF1 and 2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho; however, CNF1 and the CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria-related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal binding domain, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin. Monoclonal antibody JC4 recognizes an epitope between amino acids 169 to 191 of the N-terminal binding domain. JC4 neutralizes only CNF1. |
Information sourced from Uniprot.org