• Immunofluorescence analysis of U-2 OS cells using CLUH Polyclonal Antibody (STJ112179) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using CLUH Polyclonal Antibody (STJ112179) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using CLUH antibody (STJ112179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using CLUH antibody (STJ112179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using CLUH antibody (STJ112179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CLUH antibody (STJ112179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-CLUH antibody (1120-1309) (STJ112179)

SKU:
STJ112179

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLUH (1120-1309) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLUH
Gene ID: 23277
Uniprot ID: CLU_HUMAN
Immunogen Region: 1120-1309
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1120-1309 of human CLUH (NP_056044.3).
Immunogen Sequence: YDLSLRFLENALAVSTKYHG PKALKVALSHHLVARVYESK AEFRSALQHEKEGYTIYKTQ LGEDHEKTKESSEYLKCLTQ QAVALQRTMNEIYRNGSSAN IPPLKFTAPSMASVLEQLNV INGILFIPLSQKDLENLKAE VARRHQLQEASRNRDRAEEP MATEPAPAGAPGDLGSQPPA AKDPSPSVQG
Function mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria. Specifically binds mRNAs of nuclear-encoded mitochondrial proteins in the cytoplasm and regulates transport or translation of these transcripts close to mitochondria, playing a role in mitochondrial biogenesis.
Protein Name Clustered Mitochondria Protein Homolog
Cellular Localisation Cytoplasm
Cytoplasmic Granule
A Fraction Colocalizes With Tyrosinated Tubulin And Can Be Detected Close To Mitochondria
Alternative Antibody Names Anti-Clustered Mitochondria Protein Homolog antibody
Anti-CLUH antibody
Anti-KIAA0664 antibody

Information sourced from Uniprot.org

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