| Host: | Goat |
| Applications: | WB/IF |
| Reactivity: | Human/Rat/Mouse/Monkey/Canine |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Goat polyclonal antibody anti-Clathrin-H Chain (50aa to N-Term) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 2 mg/ml |
| Dilution Range: | WB 1:250-1:1000IF 1:25-1:250Imbastari F PhD Thesis Humboldt-Universität zu Berlin Germany 2020Caì Y Postnikova EN Bernbaum JG et al. J Virol. 2015 Jan 1:89 (1:844-56. PMID: 25355889 |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: | CLTC |
| Gene ID: | 1213 |
| Uniprot ID: | CLH1_HUMAN |
| Immunogen Region: | 50aa to N-Term |
| Accession Number: | ENSG00000141367 |
| Specificity: | Detects endogenous levels of total CLTC by Western blot in the whole cell lysates (MDCK, At-T20, COS-7). |
| Immunogen: | Purified recombinant peptide derived from within residues 50 aa to N-terminus of human CLTC produced in E. coli. |
| Immunogen Sequence: | M A Q I L P I R F Q E H L Q L Q N L G I N P A N I G F S T L T M E S D K F I C I R E K V G E Q A Q V V I I |
| Function | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation. |
| Protein Name | Clathrin Heavy Chain 1Clathrin Heavy Chain On Chromosome 17Clh-17 |
| Database Links | Reactome: R-HSA-168275Reactome: R-HSA-177504Reactome: R-HSA-190873Reactome: R-HSA-196025Reactome: R-HSA-2132295 Q00610-1Reactome: R-HSA-3928665Reactome: R-HSA-432720 Q00610-1Reactome: R-HSA-432722 Q00610-1Reactome: R-HSA-437239Reactome: R-HSA-5099900Reactome: R-HSA-5140745Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8866427Reactome: R-HSA-8964038Reactome: R-HSA-9013420Reactome: R-HSA-9013424Reactome: R-HSA-9700645Reactome: R-HSA-9725370Reactome: R-HSA-9725371 |
| Cellular Localisation | Cytoplasmic Vesicle MembranePeripheral Membrane ProteinCytoplasmic SideMembraneCoated PitMelanosomeCytoplasmCytoskeletonSpindleCytoplasmic Face Of Coated Pits And VesiclesIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvIn Complex With Tacc3 And Ckap5 (Forming The Tacc3/Ch-Tog/Clathrin Complex) Localized To Inter-Microtubule Bridges In Mitotic Spindles |
| Alternative Antibody Names | Anti-Clathrin Heavy Chain 1 antibodyAnti-Clathrin Heavy Chain On Chromosome 17 antibodyAnti-Clh-17 antibodyAnti-CLTC antibodyAnti-CLH17 antibodyAnti-CLTCL2 antibodyAnti-KIAA0034 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
