| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CLSTN3 (730-850) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CLSTN3 |
| Gene ID: |
9746 |
| Uniprot ID: |
CSTN3_HUMAN |
| Immunogen Region: |
730-850 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 730-850 of human CLSTN3 (NP_055533.2). |
| Immunogen Sequence: |
SLQQRGLELTNTSAYLTIAG VESITVYEEILRQARYRLRH GAALYTRKFRLSCSEMNGRY SSNEFIVEVNVLHSMNRVAH PSHVLSSQQFLHRGHQPPPE MAGHSLASSHRNSMIPSAAT L |
| Tissue Specificity | expressed predominantly in the brain and in kidney. Low levels in heart, skeletal muscle, liver, placenta, pancreas and lung. predominant expression in brain, and only marginal in kidney. In brain, present throughout all cortical layers, highest levels in GABAergic neurons (based on morphology and distribution pattern). Isoform CLSTN3beta: Expression is restricted to adipose tissue, with high expression in multilocular thermogenic adipocytes (brown adipose tissue). |
| Post Translational Modifications | Proteolytically processed under normal cellular conditions. A primary zeta-cleavage generates a large extracellular (soluble) N-terminal domain (sAlc) and a short C-terminal transmembrane fragment (CTF1). A secondary cleavage catalyzed by gamma-secretase within the transmembrane domain releases the beta-Alc-beta chain in the extracellular milieu and produces an intracellular fragment (AlcICD). This processing is strongly suppressed in the tripartite complex formed with APBA2 and APP, which seems to prevent the association with gamma-secretase. Isoform CLSTN3beta: Ubiquitinated: endoplasmic reticulum-localized protein is ubiquitinated and degraded by the endoplasmic reticulum-associated degradation (ERAD) pathway. |
| Function | Postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation. Promotes synapse development by acting as a cell adhesion molecule at the postsynaptic membrane, which associates with both neurexin-alpha and neurexin-beta proteins at the presynaptic membrane. Regulates the balance between excitatory and inhibitory synapses by inhibiting formation of excitatory parallel-fiber synapses and promoting formation of inhibitory synapses in the same neuron. May also be involved in ascorbate (vitamin C) uptake via its interaction with SLC23A2/SVCT2. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation (Probable). Isoform CLSTN3beta: Adipose-specific isoform that plays a key role in adaptive thermogenesis. Facilitates the efficient use of stored triglyceride by promoting multilocular morphology of thermogenic adipocytes: acts by inhibiting the activity of CIDEA and CIDEC on lipid droplets, thereby preventing lipid droplet fusion and facilitating lipid utilization. May also participate in adaptive thermogenesis by promoting sympathetic innervation of thermogenic adipose tissue: acts by driving secretion of neurotrophic factor S100B from brown adipocytes, stimulating neurite outgrowth from sympathetic neurons. |
| Protein Name | Calsyntenin-3Alcadein-BetaAlc-Beta |
| Cellular Localisation | Postsynaptic Cell MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum MembraneGolgi Apparatus MembraneCell ProjectionDendriteMost Prominent In The Postsynaptic Specializations Of Asymmetric (Type I) Synapses With Both Axodendritic And Axospinous LocalizationIsoform Clstn3beta: Lipid DropletSingle-Pass Membrane ProteinLocalizes To Endoplasmic Reticulum-Lipid Droplet Contact Sites Through The Partitioning Of Its N-Terminal Hydrophobic Hairpins Onto Lipid Droplets While Its C-Terminal Transmembrane Domain Remains Anchored In The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Calsyntenin-3 antibodyAnti-Alcadein-Beta antibodyAnti-Alc-Beta antibodyAnti-CLSTN3 antibodyAnti-CS3 antibodyAnti-KIAA0726 antibody |
Information sourced from Uniprot.org
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