| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CLPP (57-277) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CLPP |
| Gene ID: |
8192 |
| Uniprot ID: |
CLPP_HUMAN |
| Immunogen Region: |
57-277 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 57-277 of human CLPP (NP_006003.1). |
| Immunogen Sequence: |
PLIPIVVEQTGRGERAYDIY SRLLRERIVCVMGPIDDSVA SLVIAQLLFLQSESNKKPIH MYINSPGGVVTAGLAIYDTM QYILNPICTWCVGQAASMGS LLLAAGTPGMRHSLPNSRIM IHQPSGGARGQATDIAIQAE EIMKLKKQLYNIYAKHTKQS LQVIESAMERDRYMSPMEAQ EFGILDKVLVHPPQDGEDEP TLVQKEPVEAAPAAEPVPAS T |
| Tissue Specificity | Detected in liver (at protein level). Predominantly expressed in skeletal muscle. Intermediate levels in heart, liver and pancreas. Low in brain, placenta, lung and kidney. |
| Function | Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Cleaves PINK1 in the mitochondrion. |
| Protein Name | Atp-Dependent Clp Protease Proteolytic Subunit - MitochondrialEndopeptidase Clp |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Atp-Dependent Clp Protease Proteolytic Subunit - Mitochondrial antibodyAnti-Endopeptidase Clp antibodyAnti-CLPP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and CLPP knockout (KO) 293T cells, using [KO Validated] CLPP Rabbit polyclonal antibody (STJ11100851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89785/358257/STJ11100851_1__37533.1713122595.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CLPP knockout (KO) 293T cells, using [KO Validated] CLPP Rabbit polyclonal antibody (STJ11100851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
