• Immunofluorescence analysis of NIH/3T3 cells using CLPP Rabbit monoclonal antibody (STJ11101838) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon using CLPP Rabbit monoclonal antibody (STJ11101838) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using CLPP Rabbit monoclonal antibody (STJ11101838) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CLPP Rabbit monoclonal antibody (STJ11101838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CLPP antibody (178-277) [S8MR] (STJ11101838)

SKU:
STJ11101838

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CLPP (178-277) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLPP
Gene ID: 8192
Uniprot ID: CLPP_HUMAN
Immunogen Region: 178-277
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 178-277 of human CLPP (Q16740).
Immunogen Sequence: HQPSGGARGQATDIAIQAEE IMKLKKQLYNIYAKHTKQSL QVIESAMERDRYMSPMEAQE FGILDKVLVHPPQDGEDEPT LVQKEPVEAAPAAEPVPAST
Tissue Specificity Detected in liver (at protein level). Predominantly expressed in skeletal muscle. Intermediate levels in heart, liver and pancreas. Low in brain, placenta, lung and kidney.
Function Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Cleaves PINK1 in the mitochondrion.
Protein Name Atp-Dependent Clp Protease Proteolytic Subunit - Mitochondrial
Endopeptidase Clp
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Atp-Dependent Clp Protease Proteolytic Subunit - Mitochondrial antibody
Anti-Endopeptidase Clp antibody
Anti-CLPP antibody

Information sourced from Uniprot.org

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