• Western blot analysis of extracts of various cell lines, using CLEC5A antibody (STJ23169) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CLEC5A antibody (29-188) (STJ23169)

SKU:
STJ23169

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLEC5A (29-188) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLEC5A
Gene ID: 23601
Uniprot ID: CLC5A_HUMAN
Immunogen Region: 29-188
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-188 of human CLEC5A (NP_037384.1).
Immunogen Sequence: QIFNKSNDGFTTTRSYGTVS QIFGSSSPSPNGFITTRSYG TVCPKDWEFYQARCFFLSTS ESSWNESRDFCKGKGSTLAI VNTPEKLKFLQDITDAEKYF IGLIYHREEKRWRWINNSVF NGNVTNQNQNFNCATIGLTK TFDAASCDISYRRICEKNAK
Tissue Specificity Highly expressed in bone marrow with lower levels in synovium, lung and bronchus. Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U-937 and Mono-Mac-6, but not in cell lines of other origins. Expression is down-regulated when monocytes differentiate into dendritic cells.
Post Translational Modifications N-glycosylated. Contains sialic acid residues.
Function Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. (Microbial infection) Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through the phosphorylation of TYROBP. This interaction does not result in viral entry, but stimulates pro-inflammatory cytokine release.
Protein Name C-Type Lectin Domain Family 5 Member A
C-Type Lectin Superfamily Member 5
Myeloid Dap12-Associating Lectin 1
Mdl-1
Database Links Reactome: R-HSA-2172127
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-C-Type Lectin Domain Family 5 Member A antibody
Anti-C-Type Lectin Superfamily Member 5 antibody
Anti-Myeloid Dap12-Associating Lectin 1 antibody
Anti-Mdl-1 antibody
Anti-CLEC5A antibody
Anti-CLECSF5 antibody
Anti-MDL1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance