• Immunofluorescence analysis of Mouse leg bone tissue tissue using CLEC12A Rabbit polyclonal antibody (STJ28006) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of lysates from K-562 cells, using CLEC12A Rabbit polyclonal antibody (STJ28006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-CLEC12A antibody (66-265) (STJ28006)

SKU:
STJ28006

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLEC12A (66-265) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLEC12A
Gene ID: 160364
Uniprot ID: CL12A_HUMAN
Immunogen Region: 66-265
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 66-265 of human CLEC12A (NP_612210.4).
Immunogen Sequence: VTLKIEMKKMNKLQNISEEL QRNISLQLMSNMNISNKIRN LSTTLQTIATKLCRELYSKE QEHKCKPCPRRWIWHKDSCY FLSDDVQTWQESKMACAAQN ASLLKINNKNALEFIKSQSR SYDYWLGLSPEEDSTRGMRV DNIINSSAWVIRNAPDLNNM YCGYINRLYVQYYHCTYKKR MICEKMANPVQLGSTYFREA
Tissue Specificity Detected in normal myeloid cells and in acute myeloid leukemia cells. Detected in neutrophils, eosinophils, monocytes and dendritic cells. Detected in spleen macrophage-rich red pulp and in lymph node (at protein level). Detected in peripheral blood leukocytes, dendritic cells, bone marrow, monocytes, mononuclear leukocytes and macrophages.
Post Translational Modifications Highly N-glycosylated. Glycosylation varies between cell types.
Function Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases.
Protein Name C-Type Lectin Domain Family 12 Member A
C-Type Lectin-Like Molecule 1
Cll-1
Dendritic Cell-Associated Lectin 2
Dcal-2
Myeloid Inhibitory C-Type Lectin-Like Receptor
Micl
Cd Antigen Cd371
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Ligand Binding Leads To Internalization
Alternative Antibody Names Anti-C-Type Lectin Domain Family 12 Member A antibody
Anti-C-Type Lectin-Like Molecule 1 antibody
Anti-Cll-1 antibody
Anti-Dendritic Cell-Associated Lectin 2 antibody
Anti-Dcal-2 antibody
Anti-Myeloid Inhibitory C-Type Lectin-Like Receptor antibody
Anti-Micl antibody
Anti-Cd Antigen Cd371 antibody
Anti-CLEC12A antibody
Anti-CLL1 antibody
Anti-DCAL2 antibody
Anti-MICL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance