• Western blot analysis of lysates from 293T GSDMB stable cells and 293T GSDMB stable cells transfected with GZMA using Cleaved Gasdermin B (C Terminal) Rabbit monoclonal antibody (STJ11105311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. western blot samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS
  • Western blot analysis of lysates from GSDMB-C-6-his protein, using Cleaved Gasdermin B (C Terminal) Rabbit monoclonal antibody (STJ11105311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 3s. western blot samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Anti-Cleaved-GSDMB antibody (C-Term) [S5311RM] (STJ11105311)

SKU:
STJ11105311

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cleaved-Gasdermin-B (C-Term) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5311RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSDMB
Gene ID: 55876
Uniprot ID: GSDMB_HUMAN
Immunogen Region: C-Term
Immunogen: A synthetic peptide corresponding to residues surrounding Ser245 of human Gasdermin B (NP_001159430.1).
Immunogen Sequence: GKSLG
Tissue Specificity In the gastrointestinal tract, expressed in proliferating cells, including in the basal cell layer of esophagus and in isthmus/neck of stomach.
Post Translational Modifications Cleavage by granzyme A (GZMA) relieves autoinhibition by releasing the N-terminal moiety (Gasdermin-B, N-terminal) that initiates pyroptosis. Not cleaved by other granzymes. Major cleavage site takes places after Lys-244.a minor cleavage site takes place after Lys-229. Cleavage by neutrophil elastase ELANE, inhibits its ability to trigger pyroptosis. (Microbial infection) Ubiquitinated by S.flexneri IpaH7.8, leading to its degradation by the proteasome, thereby preventing its ability to form pores in bacterial-derived membranes.
Function Gasdermin-B: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death. This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-B, N-terminal) binds to membranes and forms pores, triggering pyroptosis. Also acts as a regulator of epithelial cell repair independently of programmed cell death: translocates to the plasma membrane and promotes epithelial maintenance and repair by regulating PTK2/FAK-mediated phosphorylation of PDGFA. Gasdermin-B, N-terminal: Pore-forming protein produced by cleavage by granzyme A (GZMA), which causes membrane permeabilization and pyroptosis in target cells of cytotoxic T and natural killer (NK) cells. Key downstream mediator of granzyme-mediated cell death: (1) granzyme A (GZMA), delivered to target cells from cytotoxic T- and NK-cells, (2) specifically cleaves Gasdermin-B to generate this form. After cleavage, moves to the plasma membrane, homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis. The different isoforms recognize and bind different phospholipids on membranes, promoting cell death of different target cells. Isoform 4: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death and mediates pyroptosis. Following cleavage and activation by granzyme A (GZMA), the N-terminal part binds to membrane inner leaflet lipids, homooligomerizes within the human plasma membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis. Recognizes and binds membrane inner leaflet lipids of human cells, such as phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, and more weakly to phosphatidic acid. Also binds sufatide, a component of the apical membrane of epithelial cells. Isoform 6: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death and mediates pyroptosis of human cells. Following cleavage and activation by granzyme A (GZMA), the N-terminal part binds to membrane inner leaflet lipids, homooligomerizes within the human plasma membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis. Isoform 1: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death and specifically mediates cell death of Gram-negative bacteria in response to infection. Following cleavage and activation by granzyme A (GZMA), the N-terminal part recognizes and binds phospholipids found on Gram-negative bacterial membranes, such as lipid A and cariolipin, homooligomerizes within the bacterial membranes and forms pores, triggering pyroptosis followed by cell death. In contrast to isoform 4, does not bind to membrane inner leaflet lipids of host human cell, such as phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate. Isoform 2: Not able to trigger pyroptosis. Isoform 3: Not able to trigger pyroptosis.
Protein Name Gasdermin-B
Gasdermin-Like Protein Cleaved Into - Gasdermin-B - N-Terminal
Gsdmb-Nt
P30 - Gasdermin-B - C-Terminal
Gsdmb-Ct
P16
Cellular Localisation Gasdermin-B: Cytoplasm
Vesicular Localization In The Apical Region Of Gastric Chief Cells And Colonic Surface Mucous Cells
And The Basal Region Of Neuroendocrine Cells
Gasdermin-B
N-Terminal: Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Gasdermin-B antibody
Anti-Gasdermin-Like Protein Cleaved Into - Gasdermin-B - N-Terminal antibody
Anti-Gsdmb-Nt antibody
Anti-P30 - Gasdermin-B - C-Terminal antibody
Anti-Gsdmb-Ct antibody
Anti-P16 antibody
Anti-GSDMB antibody
Anti-GSDML antibody
Anti-PP4052 antibody
Anti-PRO2521 antibody

Information sourced from Uniprot.org

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