| Short Description : | Rabbit polyclonal anti-Cleaved-DNA fragmentation factor subunit alpha-Thr225 (224-225) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range: | WB 1:1000-2000ELISA 1:5000-20000 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DFFA |
| Gene ID: | 1676 |
| Uniprot ID: | DFFA_HUMAN |
| Immunogen: | Synthesized peptide derived from human DFF45 (Cleaved-Thr225) |
| Immunogen Region: | 224-225 |
| Specificity: | This antibody detects endogenous levels of Human DFF45 (Cleaved-Thr225, protein was cleaved amino acid sequence between 224-225 ) |
| Post Translational Modifications | Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. |
| Function | Inhibitor of the caspase-activated DNase (DFF40). |
| Protein Name | Dna Fragmentation Factor Subunit AlphaDna Fragmentation Factor 45 Kda SubunitDff-45Inhibitor Of CadIcad |
| Database Links | Reactome: R-HSA-140342 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Dna Fragmentation Factor Subunit Alpha antibodyAnti-Dna Fragmentation Factor 45 Kda Subunit antibodyAnti-Dff-45 antibodyAnti-Inhibitor Of Cad antibodyAnti-Icad antibodyAnti-DFFA antibodyAnti-DFF1 antibodyAnti-DFF45 antibodyAnti-H13 antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of HeLa cells using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/102903/392132/STJ29835_1__60621.1713162515.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")