Anti-CLCNKA antibody (470-644) (STJ23154)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CLCNKA (470-644) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CLCNKA |
| Gene ID | 1187 |
| Uniprot ID | CLCKA_HUMAN |
| Immunogen Region | 470-644 |
| Immunogen Sequence | QSCQPSFYDGTIIVKKLPYL PRILGRNIGSHHVRVEHFMN HSITTLAKDTPLEEVVKVVT STDVTEYPLVESTESQILVG IVQRAQLVQALQAEPPSRAP GHQQCLQDILARGCPTEPVT LTLFSETTLHQAQNLFKLLN LQSLFVTSRGRAVGCVSWVE MKKAISNLTNPPAPK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 470-644 of human CLCNKA (NP_001244068.1). |
Additional Info
| Function | Anion-selective channel permeable to small monovalent anions with ion selectivity for chloride > bromide > nitrate > iodide. Forms a homodimeric channel where each subunit has its own ion conduction pathway. May conduct double-barreled currents controlled by two types of gates, two fast gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Assembles with the regulatory subunit BSND/Barttin for sorting at the basolateral plasma membrane domain and functional switch to the ion conducting state. CLCNKA:BSND channels display mostly a linear current-voltage relationship with fast gating at negative potentials. Mediates transepithelial chloride transport from the lumen to interstitial compartment along the thin ascending limb of Henle's loop, contributing to generation of hypertonic medullary interstitium as a countercurrent system to achieve urine concentration. Conducts chloride currents in the stria vascularis of the inner ear to establish the endocochlear potential necessary for normal hearing. |
| Protein Name | Chloride Channel Protein Clc-KaChloride Channel KaClc-K1 |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Basolateral Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Chloride Channel Protein Clc-Ka antibodyAnti-Chloride Channel Ka antibodyAnti-Clc-K1 antibodyAnti-CLCNKA antibody |
Information sourced from Uniprot.org