• Immunohistochemistry analysis of paraffin-embedded rat skeletal muscle using CLCN1 Rabbit polyclonal antibody (STJ28306) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using CLCN1 Rabbit polyclonal antibody (STJ28306) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse skeletal muscle, using CLCN1 antibody (STJ28306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CLCN1 antibody (779-988) (STJ28306)

SKU:
STJ28306

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLCN1 (779-988) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLCN1
Gene ID: 1180
Uniprot ID: CLCN1_HUMAN
Immunogen Region: 779-988
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 779-988 of human CLCN1 (NP_000074.3).
Immunogen Sequence: ARPTKKKTTQDSTDLVDNMS PEEIEAWEQEQLSQPVCFDS CCIDQSPFQLVEQTTLHKTH TLFSLLGLHLAYVTSMGKLR GVLALEELQKAIEGHTKSGV QLRPPLASFRNTTSTRKSTG APPSSAENWNLPEDRPGATG TGDVIAASPETPVPSPSPEP PLSLAPGKVEGELEELELVE SPGLEEELADILQGPSLRST DEEDEDELIL
Tissue Specificity Predominantly expressed in skeletal muscles.
Function Voltage-gated chloride channel. Plays an important role in membrane repolarization in skeletal muscle cells after muscle contraction. The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (Probable). The absence of conserved gating glutamate residues is typical for family members that function as channels (Probable).
Protein Name Chloride Channel Protein 1
Clc-1
Chloride Channel Protein - Skeletal Muscle
Database Links Reactome: R-HSA-2672351
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Chloride Channel Protein 1 antibody
Anti-Clc-1 antibody
Anti-Chloride Channel Protein - Skeletal Muscle antibody
Anti-CLCN1 antibody
Anti-CLC1 antibody

Information sourced from Uniprot.org

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