• Western blot analysis of extracts of mouse skeletal muscle, using CKM antibody (STJ26196) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of mouse heart cells using CKM rabbit polyclonal antibody (STJ26196) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of rat heart cells using CKM rabbit polyclonal antibody (STJ26196) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-CKM antibody (3-381) (STJ26196)

SKU:
STJ26196

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CKM (3-381) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:20-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CKM
Gene ID: 1158
Uniprot ID: KCRM_HUMAN
Immunogen Region: 3-381
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 3-381 of human CKM (NP_001815.2).
Immunogen Sequence: FGNTHNKFKLNYKPEEEYPD LSKHNNHMAKVLTLELYKKL RDKETPSGFTVDDVIQTGVD NPGHPFIMTVGCVAGDEESY EVFKELFDPIISDRHGGYKP TDKHKTDLNHENLKGGDDLD PNYVLSSRVRTGRSIKGYTL PPHCSRGERRAVEKLSVEAL NSLTGEFKGKYYPLKSMTEK EQQQLIDDHFLFDKPVSPLL LASGMARDWPDARGIWHNDN KSFLVWVNEEDHLRVISME
Function Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Protein Name Creatine Kinase M-Type
Creatine Kinase M Chain
Creatine Phosphokinase M-Type
Cpk-M
M-Ck
Database Links Reactome: R-HSA-71288
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Creatine Kinase M-Type antibody
Anti-Creatine Kinase M Chain antibody
Anti-Creatine Phosphokinase M-Type antibody
Anti-Cpk-M antibody
Anti-M-Ck antibody
Anti-CKM antibody
Anti-CKMM antibody

Information sourced from Uniprot.org

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