| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human/Mouse/Rat/Dog/Pig/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-CKB/Brain Creatine Kinase (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 45-48kDa band observed in human brain lysates (calculated MW of 42.6kDa according to NP_001814.2). Recommended concentration: 0.1-0.3µg/ml. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
CKB |
| Gene ID: |
1152 |
| Uniprot ID: |
KCRB_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_001814.2 |
| Immunogen Sequence: |
KPSDEHKTDLNPDN |
| Function | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work. |
| Protein Name | Creatine Kinase B-TypeBrain Creatine KinaseB-CkCreatine Kinase B ChainCreatine Phosphokinase B-TypeCpk-B |
| Database Links | Reactome: R-HSA-71288Reactome: R-HSA-9696264 |
| Cellular Localisation | CytoplasmCytosolMitochondrionCell MembraneLocalizes To The Mitochondria Of Thermogenic Fat Cells Via The Internal Mts-Like Signal (Imts-L) Region |
| Alternative Antibody Names | Anti-Creatine Kinase B-Type antibodyAnti-Brain Creatine Kinase antibodyAnti-B-Ck antibodyAnti-Creatine Kinase B Chain antibodyAnti-Creatine Phosphokinase B-Type antibodyAnti-Cpk-B antibodyAnti-CKB antibodyAnti-CKBB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
