| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CKAP4 |
| Gene ID: | 10970 |
| Uniprot ID: | CKAP4_HUMAN |
| Immunogen Region: | 130-400 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-400 of human CKAP4 (NP_006816.2). |
| Immunogen Sequence: | VLEEVQQVRRSHQDFSRQRE ELGQGLQGVEQKVQSLQATF GTFESILRSSQHKQDLTEKA VKQGESEVSRISEVLQKLQN EILKDLSDGIHVVKDARERD FTSLENTVEERLTELTKSIN DNIAIFTEVQKRSQKEINDM KAKVASLEESEGNKQDLKAL KEAVKEIQTSAKSREWDMEA LRSTLQTMESDIYTEVRELV SLKQEQQAFKEAADTERLAL QALTEKLLRSEESVSRLPE |
| Post Translational Modifications | Reversibly palmitoylated. Palmitoylation at Cys-100 by ZDHHC2 is required for its trafficking from the ER to the plasma membrane and for its perinuclear localization. Palmitoylation by ZDHHC2 is also required for its function in APF-mediated antiproliferative signaling. Increased phosphorylation during mitosis prevents binding to microtubules. |
| Function | Mediates the anchoring of the endoplasmic reticulum to microtubules. High-affinity epithelial cell surface receptor for the FZD8-related low molecular weight sialoglycopeptide APF/antiproliferative factor. Mediates the APF antiproliferative signaling within cells. |
| Protein Name | Cytoskeleton-Associated Protein 463-Kda Cytoskeleton-Linking Membrane ProteinClimp-63P63 |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-5683826Reactome: R-HSA-6798695Reactome: R-HSA-8957275Reactome: R-HSA-9696264Reactome: R-HSA-9696270 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane ProteinCell MembraneCytoplasmCytoskeletonPerinuclear RegionTranslocates To The Perinuclear Region Upon Apf-Stimulation |
| Alternative Antibody Names | Anti-Cytoskeleton-Associated Protein 4 antibodyAnti-63-Kda Cytoskeleton-Linking Membrane Protein antibodyAnti-Climp-63 antibodyAnti-P63 antibodyAnti-CKAP4 antibody |
Information sourced from Uniprot.org