| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CISD1 (29-108) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CISD1 |
| Gene ID: |
55847 |
| Uniprot ID: |
CISD1_HUMAN |
| Immunogen Region: |
29-108 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 29-108 of human CISD1 (NP_060934.1). |
| Immunogen Sequence: |
LAYKRFYVKDHRNKAMINLH IQKDNPKIVHAFDMEDLGDK AVYCRCWRSKKFPFCDGAHT KHNEETGDNVGPLIIKKKET |
| Tissue Specificity | Expression is reduced in cells derived from cystic fibrosis patients. |
| Post Translational Modifications | Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30. |
| Function | L-cysteine transaminase that catalyzes the reversible transfer of the amino group from L-cysteine to the alpha-keto acid 2-oxoglutarate to respectively form 2-oxo-3-sulfanylpropanoate and L-glutamate. The catalytic cycle occurs in the presence of pyridoxal 5'-phosphate (PLP) cofactor that facilitates transamination by initially forming an internal aldimine with the epsilon-amino group of active site Lys-55 residue on the enzyme (PLP-enzyme aldimine), subsequently displaced by formation of an external aldimine with the substrate amino group (PLP-L-cysteine aldimine). The external aldimine is further deprotonated to form a carbanion intermediate, which in the presence of 2-oxoglutarate regenerates PLP yielding final products 2-oxo-3-sulfanylpropanoate and L-glutamate. The proton transfer in carbanion intermediate is suggested to be controlled by the active site lysine residue, whereas PLP stabilizes carbanion structure through electron delocalization, also known as the electron sink effect. Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation. May be involved in iron-sulfur cluster shuttling and/or in redox reactions. Can transfer the 2Fe-2S cluster to an apo-acceptor protein only when in the oxidation state, likely serving as a redox sensor that regulates mitochondrial iron-sulfur cluster assembly and iron trafficking upon oxidative stress. |
| Protein Name | Cdgsh Iron-Sulfur Domain-Containing Protein 1Cysteine Transaminase Cisd1Mitoneet |
| Cellular Localisation | Mitochondrion Outer MembraneSingle-Pass Type Iii Membrane Protein |
| Alternative Antibody Names | Anti-Cdgsh Iron-Sulfur Domain-Containing Protein 1 antibodyAnti-Cysteine Transaminase Cisd1 antibodyAnti-Mitoneet antibodyAnti-CISD1 antibodyAnti-C10orf70 antibodyAnti-ZCD1 antibodyAnti-MDS029 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
