Host: | Goat |
Applications: | Pep-ELISA/WB |
Reactivity: | Human/Mouse/Rat/Dog/Cow |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Goat polyclonal antibody anti-CIRBP, Biotinylated (161-172) is suitable for use in ELISA and Western Blot research applications. |
Clonality: | Polyclonal |
Conjugation: | Biotin |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 18kDa band observed in Rat Testis lysates (calculated MW of 18.6kDa according to Rat NP_112409.2). See non-biotinylated parental product's datasheet for further QC data. Recomme |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | CIRBP |
Gene ID: | 1153 |
Uniprot ID: | CIRBP_HUMAN |
Immunogen Region: | 161-172 |
Accession Number: | NP_001271.1 |
Specificity: | This antibody is expected to recognize reported isoform 1 (NP_001271.1) only. |
Immunogen Sequence: | RDSYDSYATHNE. |
Post Translational Modifications | Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs. Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation. |
Function | Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor. Promotes assembly of stress granules (SGs), when overexpressed. |
Protein Name | Cold-Inducible Rna-Binding Protein A18 Hnrnp Glycine-Rich Rna-Binding Protein Cirp |
Cellular Localisation | Nucleus Nucleoplasm Cytoplasm Translocates From The Nucleus To The Cytoplasm After Exposure To Uv Radiation Translocates From The Nucleus To The Cytoplasm Into Stress Granules Upon Various Cytoplasmic Stresses Such As Osmotic And Heat Shocks Its Recruitment Into Stress Granules Occurs In The Absence Of Tiar Proteins |
Alternative Antibody Names | Anti-Cold-Inducible Rna-Binding Protein antibody Anti-A18 Hnrnp antibody Anti-Glycine-Rich Rna-Binding Protein Cirp antibody Anti-CIRBP antibody Anti-A18HNRNP antibody Anti-CIRP antibody |
Information sourced from Uniprot.org