• Immunofluorescence analysis of paraffin-embedded Mouse kidney tissue using CHUK Rabbit polyclonal antibody (STJ11105593) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of HeLa cells using CHUK Rabbit polyclonal antibody (STJ11105593) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CHUK antibody (400-660) (STJ11105593)

SKU:
STJ11105593

Current Stock:
Host: Rabbit
Applications: IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 400-660
Specificity: Recombinant Protein corresponding to a sequence within amino acids 400-660 of rat CHUK (NP_001101058.1).
Immunogen Sequence: SRSLSDCVNYIVQDSKIQLP VTQLRKVWAEAVHYVSGLKE DYSRLFQGQRAAMLSLLRYN ANLTKMKNTLISASQQLKAK LEFFRKSIQLDLERYSEQMT YGISSEKMLKAWKEMEEKAI HYSEVGVIGYLEDQIMSLHT EIMELQKSPYGRRQGDLMES LEQRAIDLYKQLKHRPTDHS YSDSTEMVKIIVHTVQSQDR VLKELFGHLSKLLGCKQKII DLLPKVEVALSNIKEADNT
Background Contributes to IkappaB kinase activity. Involved in several processes, including Rho protein signal transduction; response to cholecystokinin; and response to lipopolysaccharide. Located in cytosol and intracellular membrane-bounded organelle. Part of IkappaB kinase complex. Used to study muscular atrophy. Biomarker of colitis. Human ortholog (s) of this gene implicated in fetal encasement syndrome and prostate cancer. Orthologous to human CHUK (component of inhibitor of nuclear factor kappa B kinase complex).

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance