• STJ70936 (0. 1µg/ml) staining of isoform 1+ 2 Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70936 (2µg/ml) staining of isoform 1 Human Brain (A) and isoform 1+2 (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70936 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Chromogranin A precursor antibody (Internal) (STJ70936)

SKU:
STJ70936-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Chromogranin A precursor (Internal) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3.75µg/ml
FC-Flow cytometric analysis of A549 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:4000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CHGA
Gene ID: 1113
Uniprot ID: CMGA_HUMAN
Immunogen Region: Internal
Accession Number: NP_001266.1; NP_001288619.1
Immunogen Sequence: QGAKERAHQQKKH
Post Translational Modifications Sulfated on tyrosine residues and/or contains sulfated glycans. O-glycosylated with core 1 or possibly core 8 glycans. Contains chondroitin sulfate (CS).CS attachment is pH-dependent, being observed at mildly acidic conditions of pH 5 but not at neutral pH, and promotes self-assembly in vitro. Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity. Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides.
Function Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. Catestatin: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa. Can induce mast cell migration, degranulation and production of cytokines and chemokines. Acts as a potent scavenger of free radicals in vitro. May play a role in the regulation of cardiac function and blood pressure. Serpinin: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Protein Name Chromogranin-A
Cga
Pituitary Secretory Protein I
Sp-I Cleaved Into - Vasostatin-1
Vasostatin I - Vasostatin-2
Vasostatin Ii - Ea-92 - Es-43 - Pancreastatin - Ss-18 - Wa-8 - We-14 - Lf-19 - Catestatin
Sl21 - Al-11 - Gv-19 - Gr-44 - Er-37 - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor
Database Links Reactome: R-HSA-6803157
Cellular Localisation Serpinin: Secreted
Cytoplasmic Vesicle
Secretory Vesicle
Pyroglutaminated Serpinin Localizes To Secretory Vesicle
Neuronal Dense Core Vesicle
Secreted
Associated With The Secretory Granule Membrane Through Direct Interaction To Scg3 That In Turn Binds To Cholesterol-Enriched Lipid Rafts In Intragranular Conditions
In Pituitary Gonadotropes
Located In Large Secretory Granules
Alternative Antibody Names Anti-Chromogranin-A antibody
Anti-Cga antibody
Anti-Pituitary Secretory Protein I antibody
Anti-Sp-I Cleaved Into - Vasostatin-1 antibody
Anti-Vasostatin I - Vasostatin-2 antibody
Anti-Vasostatin Ii - Ea-92 - Es-43 - Pancreastatin - Ss-18 - Wa-8 - We-14 - Lf-19 - Catestatin antibody
Anti-Sl21 - Al-11 - Gv-19 - Gr-44 - Er-37 - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor antibody
Anti-CHGA antibody

Information sourced from Uniprot.org

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