Anti-Chromogranin A antibody (1-100) [S6MR] (STJ11103666)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Chromogranin A (1-100) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S6MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:1000-1:5000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CHGA |
| Gene ID | 1113 |
| Uniprot ID | CMGA_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MRSAAVLALLLCAGQVTALP VNSPMNKGDTEVMKCIVEVI SDTLSKPSPMPVSQECFETL RGDERILSILRHQNLLKELQ DLALQGAKERAHQQKKHSGF |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Chromogranin A (P10645). |
Additional Info
| Tissue Specificity | Detected in cerebrospinal fluid (at protein level). Detected in urine (at protein level). GE-25: Found in the brain. |
| Post Translational Modifications | Sulfated on tyrosine residues and/or contains sulfated glycans. O-glycosylated with core 1 or possibly core 8 glycans. Contains chondroitin sulfate (CS).CS attachment is pH-dependent, being observed at mildly acidic conditions of pH 5 but not at neutral pH, and promotes self-assembly in vitro. Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity. Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides. |
| Function | Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. Catestatin: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa. Can induce mast cell migration, degranulation and production of cytokines and chemokines. Acts as a potent scavenger of free radicals in vitro. May play a role in the regulation of cardiac function and blood pressure. Serpinin: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation. |
| Protein Name | Chromogranin-ACgaPituitary Secretory Protein ISp-I Cleaved Into - Vasostatin-1Vasostatin I - Vasostatin-2Vasostatin Ii - Ea-92 - Es-43 - Pancreastatin - Ss-18 - Wa-8 - We-14 - Lf-19 - CatestatinSl21 - Al-11 - Gv-19 - Gr-44 - Er-37 - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor |
| Database Links | Reactome: R-HSA-6803157 |
| Cellular Localisation | Serpinin: SecretedCytoplasmic VesicleSecretory VesiclePyroglutaminated Serpinin Localizes To Secretory VesicleNeuronal Dense Core VesicleSecretedAssociated With The Secretory Granule Membrane Through Direct Interaction To Scg3 That In Turn Binds To Cholesterol-Enriched Lipid Rafts In Intragranular ConditionsIn Pituitary GonadotropesLocated In Large Secretory Granules |
| Alternative Antibody Names | Anti-Chromogranin-A antibodyAnti-Cga antibodyAnti-Pituitary Secretory Protein I antibodyAnti-Sp-I Cleaved Into - Vasostatin-1 antibodyAnti-Vasostatin I - Vasostatin-2 antibodyAnti-Vasostatin Ii - Ea-92 - Es-43 - Pancreastatin - Ss-18 - Wa-8 - We-14 - Lf-19 - Catestatin antibodyAnti-Sl21 - Al-11 - Gv-19 - Gr-44 - Er-37 - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor antibodyAnti-CHGA antibody |
Information sourced from Uniprot.org