• Western blot analysis of extracts of various cell lines, using Muscarinic AChR M2 (Muscarinic AChR M2 (ACM2) ) rabbit monoclonal antibody (STJ11102277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Muscarinic AChR M2 (Muscarinic AChR M2 (ACM2) ) rabbit monoclonal antibody (STJ11102277) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Muscarinic AChR M2 (ACM2) in paraffin-embedded human tonsil tissue using Muscarinic AChR M2 (ACM2) rabbit monoclonal antibody (STJ11102277) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Muscarinic AChR M2 (ACM2) in paraffin-embedded mouse kidney tissue using Muscarinic AChR M2 (ACM2) rabbit monoclonal antibody (STJ11102277) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Muscarinic AChR M2 (ACM2) in paraffin-embedded mouse testis tissue using Muscarinic AChR M2 (ACM2) rabbit monoclonal antibody (STJ11102277) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Muscarinic AChR M2 (ACM2) in paraffin-embedded rat brain tissue using Muscarinic AChR M2 (ACM2) rabbit monoclonal antibody (STJ11102277) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CHRM2 antibody (100-200) [S7MR] (STJ11102277)

SKU:
STJ11102277

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHRM2
Gene ID: 1129
Uniprot ID: ACM2_HUMAN
Immunogen Region: 100-200
Specificity: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Muscarinic AChR M2 (ACM2) (P08172).
Immunogen Sequence: LALDYVVSNASVMNLLIISF DRYFCVTKPLTYPVKRTTKM AGMMIAAAWVLSFILWAPAI LFWQFIVGVRTVEDGECYIQ FFSNAAVTFGTAIAAFYLPV I
Post Translational Modifications Phosphorylated in response to agonist treatment.
Function The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3).this then triggers calcium ion release into the cytosol.
Protein Name Muscarinic Acetylcholine Receptor M2
Database Links Reactome: R-HSA-390648
Reactome: R-HSA-418594
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Phosphorylation In Response To Agonist Binding Promotes Receptor Internalization
Alternative Antibody Names Anti-Muscarinic Acetylcholine Receptor M2 antibody
Anti-CHRM2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance