• Western blot analysis of lysate from A549 cells, using Chr-A antibody.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of Chr-A antibody in paraffin-embedded human brain tissue.
  • Western blot analysis of various cells using Chr-A Polyclonal Antibody

Anti-CHGA antibody (311-360 aa) (STJ92281)

SKU:
STJ92281

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Chromogranin-A (311-360 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHGA
Gene ID: 1113
Uniprot ID: CMGA_HUMAN
Immunogen Region: 311-360 aa
Specificity: Chr-A Polyclonal Antibody detects endogenous levels of Chr-A protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Chr-A at the amino acid range 311-360
Post Translational Modifications Sulfated on tyrosine residues and/or contains sulfated glycans. O-glycosylated with core 1 or possibly core 8 glycans. Contains chondroitin sulfate (CS).CS attachment is pH-dependent, being observed at mildly acidic conditions of pH 5 but not at neutral pH, and promotes self-assembly in vitro. Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity. Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides.
Function Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. Catestatin: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa. Can induce mast cell migration, degranulation and production of cytokines and chemokines. Acts as a potent scavenger of free radicals in vitro. May play a role in the regulation of cardiac function and blood pressure. Serpinin: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Protein Name Chromogranin-A
Cga
Pituitary Secretory Protein I
Sp-I Cleaved Into - Vasostatin-1
Vasostatin I - Vasostatin-2
Vasostatin Ii - Ea-92 - Es-43 - Pancreastatin - Ss-18 - Wa-8 - We-14 - Lf-19 - Catestatin
Sl21 - Al-11 - Gv-19 - Gr-44 - Er-37 - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor
Database Links Reactome: R-HSA-6803157
Cellular Localisation Serpinin: Secreted
Cytoplasmic Vesicle
Secretory Vesicle
Pyroglutaminated Serpinin Localizes To Secretory Vesicle
Neuronal Dense Core Vesicle
Secreted
Associated With The Secretory Granule Membrane Through Direct Interaction To Scg3 That In Turn Binds To Cholesterol-Enriched Lipid Rafts In Intragranular Conditions
In Pituitary Gonadotropes
Located In Large Secretory Granules
Alternative Antibody Names Anti-Chromogranin-A antibody
Anti-Cga antibody
Anti-Pituitary Secretory Protein I antibody
Anti-Sp-I Cleaved Into - Vasostatin-1 antibody
Anti-Vasostatin I - Vasostatin-2 antibody
Anti-Vasostatin Ii - Ea-92 - Es-43 - Pancreastatin - Ss-18 - Wa-8 - We-14 - Lf-19 - Catestatin antibody
Anti-Sl21 - Al-11 - Gv-19 - Gr-44 - Er-37 - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor antibody
Anti-CHGA antibody

Information sourced from Uniprot.org

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