| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Chk2 (1-220) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IF/ICC 1:50-1:200IP 1:20-1:50 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CHEK2 |
| Gene ID: |
11200 |
| Uniprot ID: |
CHK2_HUMAN |
| Immunogen Region: |
1-220 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Chk2 (NP_009125.1). |
| Immunogen Sequence: |
MSRESDVEAQQSHGSSACSQ PHGSVTQSQGSSSQSQGISS SSTSTMPNSSQSSHSSSGTL SSLETVSTQELYSIPEDQEP EDQEPEEPTPAPWARLWALQ DGFANLECVNDNYWFGRDKS CEYCFDEPLLKRTDKYRTYS KKHFRIFREVGPKNSYIAYI EDHSGNGTFVNTELVGKGKR RPLNNNSEIALSLSRNKVFV FFDLTVDDQSVYPKALRDEY |
| Tissue Specificity | High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues. |
| Post Translational Modifications | Phosphorylated. Phosphorylated at Ser-73 by PLK3 in response to DNA damage, promoting phosphorylation at Thr-68 by ATM and the G2/M transition checkpoint. Phosphorylation at Thr-68 induces homodimerization. Autophosphorylates at Thr-383 and Thr-387 in the T-loop/activation segment upon dimerization to become fully active and phosphorylate its substrates like for instance CDC25C. DNA damage-induced autophosphorylation at Ser-379 induces CUL1-mediated ubiquitination and regulates the pro-apoptotic function. Phosphorylation at Ser-456 also regulates ubiquitination. Phosphorylated by PLK4. Ubiquitinated. CUL1-mediated ubiquitination regulates the pro-apoptotic function. Ubiquitination may also regulate protein stability. Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination. |
| Function | Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence L-X-R-X-X-S/T. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition. (Microbial infection) Phosphorylates herpes simplex virus 1/HHV-1 protein ICP0 and thus activates its SUMO-targeted ubiquitin ligase activity. |
| Protein Name | Serine/Threonine-Protein Kinase Chk2Chk2 Checkpoint HomologCds1 HomologHucds1Hcds1Checkpoint Kinase 2 |
| Database Links | Reactome: R-HSA-5693565Reactome: R-HSA-6804756Reactome: R-HSA-6804757Reactome: R-HSA-6804760Reactome: R-HSA-69473Reactome: R-HSA-69541Reactome: R-HSA-69601Reactome: R-HSA-75035 |
| Cellular Localisation | Isoform 2: NucleusIsoform 10 Is Present Throughout The CellIsoform 4: NucleusIsoform 7: NucleusIsoform 9: NucleusIsoform 12: NucleusNucleusPml BodyNucleoplasmRecruited Into Pml Bodies Together With Tp53 |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Chk2 antibodyAnti-Chk2 Checkpoint Homolog antibodyAnti-Cds1 Homolog antibodyAnti-Hucds1 antibodyAnti-Hcds1 antibodyAnti-Checkpoint Kinase 2 antibodyAnti-CHEK2 antibodyAnti-CDS1 antibodyAnti-CHK2 antibodyAnti-RAD53 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
