Anti-CHD4 antibody [5F1-A11-C9] (STJ99175)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2b
ImmunogenPurified recombinant human CHD4 protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-Chromodomain-helicase-DNA-binding protein 4 for use in WB and CoIP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/CoIP |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 5F1-A11-C9 |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000IP 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CHD4 |
| Gene ID | 1108 |
| Uniprot ID | CHD4_HUMAN |
| Immunogen | Purified recombinant human CHD4 protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of CHD4 and does not cross-react with related proteins. |
Additional Info
| Function | ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination. Involved in neurogenesis. |
| Protein Name | Chromodomain-Helicase-Dna-Binding Protein 4Chd-4Atp-Dependent Helicase Chd4Mi-2 Autoantigen 218 Kda ProteinMi2-Beta |
| Database Links | Reactome: R-HSA-3214815Reactome: R-HSA-427389Reactome: R-HSA-6804758Reactome: R-HSA-73762Reactome: R-HSA-8943724Reactome: R-HSA-9031628Reactome: R-HSA-9679191Reactome: R-HSA-9843940Reactome: R-HSA-9844594Reactome: R-HSA-9845323 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeAssociates With Centrosomes In InterphaseLocalizes To Sites Of Dna Damage In A Manner Dependent On Zmynd8 And Znf687 |
| Alternative Antibody Names | Anti-Chromodomain-Helicase-Dna-Binding Protein 4 antibodyAnti-Chd-4 antibodyAnti-Atp-Dependent Helicase Chd4 antibodyAnti-Mi-2 Autoantigen 218 Kda Protein antibodyAnti-Mi2-Beta antibodyAnti-CHD4 antibody |
Information sourced from Uniprot.org