| Short Description : | Rabbit polyclonal CHCHD3 (1-227) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CHCHD3 |
| Gene ID: | 54927 |
| Uniprot ID: | MIC19_HUMAN |
| Immunogen Region: | 1-227 |
| Immunogen Sequence: | MGGTTSTRRVTFEADENENI TVVKGIRLSENVIDRMKESS PSGSKSQRYSGAYGASVSDE ELKRRVAEELALEQAKKESE DQKRLKQAKELDRERAAANE QLTRAILRERICSEEERAKA KHLARQLEEKDRVLKKQDAF YKEQLARLEERSSEFYRVTT EQYQKAAEEVEAKFKRYESH PVCADLQAKILQCYRENTHQ TLKCSALATQYMHCVNHAKQ SMLEKGG |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human CHCHD3 (NP_060282.1). |
| Tissue Specificity | Detected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue. |
| Function | Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. |
| Protein Name | Micos Complex Subunit Mic19Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 3 |
| Database Links | Reactome: R-HSA-1268020Reactome: R-HSA-8949613 |
| Cellular Localisation | Mitochondrion Inner MembraneLipid-AnchorIntermembrane SideCytoplasmNucleusMitochondrion |
| Alternative Antibody Names | Anti-Micos Complex Subunit Mic19 antibodyAnti-Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 3 antibodyAnti-CHCHD3 antibodyAnti-MIC19 antibodyAnti-MINOS3 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and CHCHD3 knockout (KO) 293T cells, using [KO Validated] CHCHD3 Rabbit polyclonal antibody (STJ11100835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89770/358242/STJ11100835_1__03090.1713122575.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CHCHD3 knockout (KO) 293T cells, using [KO Validated] CHCHD3 Rabbit polyclonal antibody (STJ11100835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
