![Immunohistochemistry analysis of paraffin-embedded mouse kidney using [KO Validated] CHCHD2 rabbit polyclonal antibody (STJ119068) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/97945/381392/STJ119068_IHC_3__17517.1713149528.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded mouse kidney using [KO Validated] CHCHD2 rabbit polyclonal antibody (STJ119068) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of paraffin-embedded Rat ovary using [KO Validated] CHCHD2 rabbit polyclonal antibody (STJ119068) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/97945/381393/STJ119068_IHC_4__93794.1713149529.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Rat ovary using [KO Validated] CHCHD2 rabbit polyclonal antibody (STJ119068) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] CHCHD2 rabbit polyclonal antibody (STJ119068) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/97945/381394/STJ119068_IHC_5__87208.1713149529.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] CHCHD2 rabbit polyclonal antibody (STJ119068) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
Anti-CHCHD2 antibody (75-145) (STJ119068)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CHCHD2 (75-145) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CHCHD2 |
| Gene ID | 51142 |
| Uniprot ID | CHCH2_HUMAN |
| Immunogen Region | 75-145 |
| Immunogen Sequence | TLGHAITGGFSGGSNAEPAR PDITYQEPQGTQPAQQQQPC LYEIKQFLECAQNQGDIKLC EGFNEVLKQCR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 75-145 of human CHCHD2 (NP_057223.1). |
Additional Info
| Function | Transcription factor. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen), as well as normoxia conditions (20% oxygen). |
| Protein Name | Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 2Aging-Associated Gene 10 ProteinHcv Ns2 Trans-Regulated ProteinNs2tp |
| Database Links | Reactome: R-HSA-1268020Reactome: R-HSA-9837999 |
| Cellular Localisation | NucleusMitochondrionMitochondrion Intermembrane SpaceMainly Localized In The Intermembrane Space |
| Alternative Antibody Names | Anti-Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 2 antibodyAnti-Aging-Associated Gene 10 Protein antibodyAnti-Hcv Ns2 Trans-Regulated Protein antibodyAnti-Ns2tp antibodyAnti-CHCHD2 antibodyAnti-C7orf17 antibodyAnti-AAG10 antibody |
Information sourced from Uniprot.org