• Immunohistochemistry analysis of CHAT in paraffin-embedded rat brain using CHAT Rabbit polyclonal antibody (STJ23113) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CHAT Rabbit polyclonal antibody (STJ23113). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CHAT antibody (200-300) (STJ23113)

SKU:
STJ23113

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHAT (200-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHAT
Gene ID: 1103
Uniprot ID: CLAT_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human CHAT (NP_065574.4).
Immunogen Sequence: DMYLNNRLALPVNSSPAVIF ARQHFPGTDDQLRFAASLIS GVLSYKALLDSHSIPTDCAK GQLSGQPLCMKQYYGLFSSY RLPGHTQDTLVAQNSSIMPE
Function Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Protein Name Choline O-Acetyltransferase
Choactase
Chat
Choline Acetylase
Database Links Reactome: R-HSA-1483191
Reactome: R-HSA-264642
Alternative Antibody Names Anti-Choline O-Acetyltransferase antibody
Anti-Choactase antibody
Anti-Chat antibody
Anti-Choline Acetylase antibody
Anti-CHAT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance