• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using p60 CAF1 Rabbit monoclonal antibody (STJ11102241) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using p60 CAF1 Rabbit monoclonal antibody (STJ11102241) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CHAF1B antibody (459-559) [S1MR] (STJ11102241)

SKU:
STJ11102241

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-p60 CAF1 (459-559) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHAF1B
Gene ID: 8208
Uniprot ID: CAF1B_HUMAN
Immunogen Region: 459-559
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 459-559 of human p60 CAF1 (Q13112).
Immunogen Sequence: PLPGPSEEKTLQPSSQNTKA HPSRRVTLNTLQAWSKTTPR RINLTPLKTDTPPSSVPTSV ISTPSTEEIQSETPGDAQGS PPELKRPRLDENKGGTESLD P
Post Translational Modifications Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
Function Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA.histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
Protein Name Chromatin Assembly Factor 1 Subunit B
Caf-1 Subunit B
Chromatin Assembly Factor I P60 Subunit
Caf-I 60 Kda Subunit
Caf-I P60
M-Phase Phosphoprotein 7
Cellular Localisation Nucleus
Cytoplasm
Dna Replication Foci
Cytoplasmic In M Phase
Alternative Antibody Names Anti-Chromatin Assembly Factor 1 Subunit B antibody
Anti-Caf-1 Subunit B antibody
Anti-Chromatin Assembly Factor I P60 Subunit antibody
Anti-Caf-I 60 Kda Subunit antibody
Anti-Caf-I P60 antibody
Anti-M-Phase Phosphoprotein 7 antibody
Anti-CHAF1B antibody
Anti-CAF1A antibody
Anti-CAF1P60 antibody
Anti-MPHOSPH7 antibody
Anti-MPP7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance