Immunohistochemistry analysis of paraffin-embedded mouse spleen using p60 CAF1 antibody (STJ28015) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CHAF1B antibody (270-559) (STJ28015)

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STJ28015
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHAF1B
Gene ID: 8208
Uniprot ID: CAF1B_HUMAN
Immunogen Region: 270-559
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 270-559 of human p60 CAF1 (NP_005432.1).
Immunogen Sequence: SRKNLKRPIAHLPCPGKATL AVRCCPVYFELRPVVETGVE LMSLPYRLVFAVASEDSVLL YDTQQSFPFGYVSNIHYHTL SDISWSSDGAFLAISSTDGY CSFVTFEKDELGIPLKEKPV LNMRTPDTAKKTKSQTHRGS SPGPRPVEGTPASRTQDPSS PGTTPPQARQAPAPTVIRDP PSITPAVKSPLPGPSEEKTL QPSSQNTKAHPSRRVTLNTL QAWSKTTPRRINLTPLKTD
Post Translational Modifications Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
Function Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA.histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
Protein Name Chromatin Assembly Factor 1 Subunit B
Caf-1 Subunit B
Chromatin Assembly Factor I P60 Subunit
Caf-I 60 Kda Subunit
Caf-I P60
M-Phase Phosphoprotein 7
Cellular Localisation Nucleus
Cytoplasm
Dna Replication Foci
Cytoplasmic In M Phase
Alternative Antibody Names Anti-Chromatin Assembly Factor 1 Subunit B antibody
Anti-Caf-1 Subunit B antibody
Anti-Chromatin Assembly Factor I P60 Subunit antibody
Anti-Caf-I 60 Kda Subunit antibody
Anti-Caf-I P60 antibody
Anti-M-Phase Phosphoprotein 7 antibody
Anti-CHAF1B antibody
Anti-CAF1A antibody
Anti-CAF1P60 antibody
Anti-MPHOSPH7 antibody
Anti-MPP7 antibody

Information sourced from Uniprot.org

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