Western blot analysis of extracts of various cell lines, using p150 CAF1 rabbit monoclonal antibody (STJ11102363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CHAF1A antibody (100-200) [S3MR] (STJ11102363)

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STJ11102363
£129.50 - £574.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHAF1A
Gene ID: 10036
Uniprot ID: CAF1A_HUMAN
Immunogen Region: 100-200
Specificity: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human p150 CAF1 (NP_005474.2).
Immunogen Sequence: NFLRNRIETSIGQSTVIIDL TEDSNEQPDSLVDHNKLNSE ASPSREAINGQREDTGDQQG LLKAIQNDKLAFPGETLSDI PCKTEEEGVGCGGAGRRGDS Q
Function Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA.histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
Protein Name Chromatin Assembly Factor 1 Subunit A
Caf-1 Subunit A
Chromatin Assembly Factor I P150 Subunit
Caf-I 150 Kda Subunit
Caf-I P150
Hp150
Cellular Localisation Nucleus
Dna Replication Foci
Alternative Antibody Names Anti-Chromatin Assembly Factor 1 Subunit A antibody
Anti-Caf-1 Subunit A antibody
Anti-Chromatin Assembly Factor I P150 Subunit antibody
Anti-Caf-I 150 Kda Subunit antibody
Anti-Caf-I P150 antibody
Anti-Hp150 antibody
Anti-CHAF1A antibody
Anti-CAF antibody
Anti-CAF1P150 antibody

Information sourced from Uniprot.org

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