• Immunofluorescence analysis of U-2 OS cells using CFP Rabbit polyclonal antibody (STJ27351) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using CFP Rabbit polyclonal antibody (STJ27351) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CFP Rabbit polyclonal antibody (STJ27351) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of Human plasma, using CFP antibody (STJ27351) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CFP antibody (190-469) (STJ27351)

SKU:
STJ27351

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFP
Gene ID: 5199
Uniprot ID: PROP_HUMAN
Immunogen Region: 190-469
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 190-469 of human CFP (NP_001138724.1).
Immunogen Sequence: CPTHGAWATWGPWTPCSASC HGGPHEPKETRSRKCSAPEP SQKPPGKPCPGLAYEQRRCT GLPPCPVAGGWGPWGPVSPC PVTCGLGQTMEQRTCNHPVP QHGGPFCAGDATRTHICNTA VPCPVDGEWDSWGEWSPCIR RNMKSISCQEIPGQQSRGRT CRGRKFDGHRCAGQQQDIRH CYSIQHCPLKGSWSEWSTWG LCMPPCGPNPTRARQRLCTP LLPKYPPTVSMVEGQGEKN
Function A positive regulator of the alternate pathway (AP) of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Inhibits CFI-CFH mediated degradation of Complement C3 beta chain (C3b).
Protein Name Properdin
Complement Factor P
Database Links Reactome: R-HSA-173736
Reactome: R-HSA-174577
Reactome: R-HSA-5083635
Reactome: R-HSA-5173214
Reactome: R-HSA-6798695
Reactome: R-HSA-977606
Cellular Localisation Secreted
Alternative Antibody Names Anti-Properdin antibody
Anti-Complement Factor P antibody
Anti-CFP antibody
Anti-PFC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance