• Confocal imaging of human liver using Ceruloplasmin Rabbit monoclonal antibody (STJ11103232, at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.
  • Immunofluorescence analysis of mouse liver using Ceruloplasmin Rabbit monoclonal antibody (STJ11103232) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat liver using Ceruloplasmin Rabbit monoclonal antibody (STJ11103232) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human liver using Ceruloplasmin Rabbit monoclonal antibody (STJ11103232) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using Ceruloplasmin Rabbit monoclonal antibody (STJ11103232) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Ceruloplasmin antibody (STJ11103232) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Ceruloplasmin antibody (366-572) [S2MR] (STJ11103232)

SKU:
STJ11103232

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:5000-1:30000
IHC-P:1:5000-1:20000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CP
Gene ID: 1356
Uniprot ID: CERU_HUMAN
Immunogen Region: 366-572
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 366-572 of human Ceruloplasmin (NP_000087.1).
Immunogen Sequence: IRGKHVRHYYIAAEEIIWNY APSGIDIFTKENLTAPGSDS AVFFEQGTTRIGGSYKKLVY REYTDASFTNRKERGPEEEH LGILGPVIWAEVGDTIRVTF HNKGAYPLSIEPIGVRFNKN NEGTYYSPNYNPQSRSVPPS ASHVAPTETFTYEWTVPKEV GPTNADPVCLAKMYYSAVDP TKDIFTGLIGPMKICKKGSL HANGRQK
Tissue Specificity Expressed by the liver and secreted in plasma.
Function Multifunctional blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Copper ions provide a large number of enzymatic activites. Oxidizes highly toxic ferrous ions to the ferric state for further incorporation onto apo-transferrins, catalyzes Cu(+) oxidation and promotes the oxidation of biogenic amines such as norepinephrin and serotonin. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. Has glutathione peroxidase-like activity, can remove both hydrogen peroxide and lipid hydroperoxide in the presence of thiols. Also shows NO-oxidase and NO2 synthase activities that determine endocrine NO homeostasis.
Protein Name Ceruloplasmin
Cuproxidase Ceruloplasmin
Ferroxidase Ceruloplasmin
Glutathione Peroxidase Ceruloplasmin
Glutathione-Dependent Peroxiredoxin Ceruloplasmin
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-425410
Reactome: R-HSA-5619049
Reactome: R-HSA-5619060
Reactome: R-HSA-8957275
Reactome: R-HSA-917937
Cellular Localisation Secreted
Colocalizes With Gcp1 In Secretory Intracellular Compartments
Alternative Antibody Names Anti-Ceruloplasmin antibody
Anti-Cuproxidase Ceruloplasmin antibody
Anti-Ferroxidase Ceruloplasmin antibody
Anti-Glutathione Peroxidase Ceruloplasmin antibody
Anti-Glutathione-Dependent Peroxiredoxin Ceruloplasmin antibody
Anti-CP antibody

Information sourced from Uniprot.org

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