• Western blot analysis of HeLa, using CENPF Rabbit polyclonal antibody (STJ11100576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CENPF antibody (1730-1890) (STJ11100576)

SKU:
STJ11100576

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CENPF (1730-1890) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CENPF
Gene ID: 1063
Uniprot ID: CENPF_HUMAN
Immunogen Region: 1730-1890
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1730-1890 of human CENPF (NP_057427.3).
Immunogen Sequence: NYEPPGEDKTQGSSECISEL SFSGPNALVPMDFLGNQEDI HNLQLRVKETSNENLRLLHV IEDRDRKVESLLNEMKELDS KLHLQEVQLMTKIEACIELE KIVGELKKENSDLSEKLEYF SCDHQELLQRVETSEGLNSD LEMHADKSSREDIGDNVAKV N
Post Translational Modifications Hyperphosphorylated during mitosis.
Function Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
Protein Name Centromere Protein F
Cenp-F
Ah Antigen
Kinetochore Protein Cenpf
Mitosin
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-156711
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Perinuclear Region
Nucleus Matrix
Chromosome
Centromere
Kinetochore
Cytoskeleton
Spindle
Relocalizes To The Kinetochore/Centromere (Coronal Surface Of The Outer Plate) And The Spindle During Mitosis
Observed In Nucleus During Interphase But Not In The Nucleolus
At Metaphase Becomes Localized To Areas Including Kinetochore And Mitotic Apparatus As Well As Cytoplasm
By Telophase
Is Concentrated Within The Intracellular Bridge At Either Side Of The Mid-Body
Alternative Antibody Names Anti-Centromere Protein F antibody
Anti-Cenp-F antibody
Anti-Ah Antigen antibody
Anti-Kinetochore Protein Cenpf antibody
Anti-Mitosin antibody
Anti-CENPF antibody

Information sourced from Uniprot.org

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