| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CENPB |
| Gene ID: | 1059 |
| Uniprot ID: | CENPB_HUMAN |
| Immunogen Region: | C-Term |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human CENPB (NP_001801.1). |
| Immunogen Sequence: | TLHFLEGGEDSDSDSEEEDD EEEDDEDEDDDDDEEDGDEV PVPSFGEAMAYFAMVKRYLT SFPIDDRVQSHILHLEHDLV HVTRKNHARQAGVRGLGHQS |
| Post Translational Modifications | Poly-ADP-ribosylated by PARP1. N-terminally methylated by METTL11A/NTM1. Alpha-N-methylation is stimulated in response to extracellular stimuli, including increased cell density and heat shock, and seems to facilitate binding to CENP-B boxes. Chromatin-bound CENP-B is primarily trimethylated. |
| Function | Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable). |
| Protein Name | Major Centromere Autoantigen BCentromere Protein BCenp-B |
| Cellular Localisation | NucleusChromosomeCentromere |
| Alternative Antibody Names | Anti-Major Centromere Autoantigen B antibodyAnti-Centromere Protein B antibodyAnti-Cenp-B antibodyAnti-CENPB antibody |
Information sourced from Uniprot.org