• Western blot analysis of extracts of various cell lines, using CELF2 antibody (STJ27812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CELF2 antibody (422-521) (STJ27812)

SKU:
STJ27812

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CELF2 (422-521) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CELF2
Gene ID: 10659
Uniprot ID: CELF2_HUMAN
Immunogen Region: 422-521
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 422-521 of human CELF2 (NP_006552.3).
Immunogen Sequence: QQSAAGSQKEGPEGANLFIY HLPQEFGDQDILQMFMPFGN VISAKVFIDKQTNLSKCFGF VSYDNPVSAQAAIQAMNGFQ IGMKRLKVQLKRSKNDSKPY
Tissue Specificity Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.
Function RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury. Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediately upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA. Binds to an intronic RNA element responsible for the silencing of exon 21 splicing. Binds to (CUG)n repeats. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF1, negatively regulates the processing to mature miRNA.
Protein Name Cugbp Elav-Like Family Member 2
Celf-2
Bruno-Like Protein 3
Cug Triplet Repeat Rna-Binding Protein 2
Cug-Bp2
Cug-Bp- And Etr-3-Like Factor 2
Elav-Type Rna-Binding Protein 3
Etr-3
Neuroblastoma Apoptosis-Related Rna-Binding Protein
Hnapor
Rna-Binding Protein Brunol-3
Cellular Localisation Nucleus
Cytoplasm
Accumulates In The Cytoplasm After Ionizing Radiation
Colocalizes With Apobec1 And A1cf
Rna-Binding Activity Is Detected In Both Nuclear And Cytoplasmic Compartments
Alternative Antibody Names Anti-Cugbp Elav-Like Family Member 2 antibody
Anti-Celf-2 antibody
Anti-Bruno-Like Protein 3 antibody
Anti-Cug Triplet Repeat Rna-Binding Protein 2 antibody
Anti-Cug-Bp2 antibody
Anti-Cug-Bp- And Etr-3-Like Factor 2 antibody
Anti-Elav-Type Rna-Binding Protein 3 antibody
Anti-Etr-3 antibody
Anti-Neuroblastoma Apoptosis-Related Rna-Binding Protein antibody
Anti-Hnapor antibody
Anti-Rna-Binding Protein Brunol-3 antibody
Anti-CELF2 antibody
Anti-BRUNOL3 antibody
Anti-CUGBP2 antibody
Anti-ETR3 antibody
Anti-NAPOR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance