• Immunofluorescence analysis of MCF7 cells using CDKN3 Rabbit polyclonal antibody (STJ23083) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse liver using CDKN3 Rabbit polyclonal antibody (STJ23083) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:60s.
  • Western blot analysis of lysates from MCF7 cells, using CDKN3 Rabbit polyclonal antibody (STJ23083) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CDKN3 antibody (1-69) (STJ23083)

SKU:
STJ23083

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDKN3 (1-69) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDKN3
Gene ID: 1033
Uniprot ID: CDKN3_HUMAN
Immunogen Region: 1-69
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-69 of human CDKN3 (NP_005183.2).
Immunogen Sequence: MKPPSSIQTSEFDSSDEEPI EDEQTPIHISWLSLSRVNCS QFLGLCALPGCKFKDVRRNV QKDTEELKS
Function May play a role in cell cycle regulation. Dual specificity CC phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner.
Protein Name Cyclin-Dependent Kinase Inhibitor 3
Cdk2-Associated Dual-Specificity Phosphatase
Cyclin-Dependent Kinase Interactor 1
Cyclin-Dependent Kinase-Interacting Protein 2
Kinase-Associated Phosphatase
Cellular Localisation Cytoplasm
Perinuclear Region
Alternative Antibody Names Anti-Cyclin-Dependent Kinase Inhibitor 3 antibody
Anti-Cdk2-Associated Dual-Specificity Phosphatase antibody
Anti-Cyclin-Dependent Kinase Interactor 1 antibody
Anti-Cyclin-Dependent Kinase-Interacting Protein 2 antibody
Anti-Kinase-Associated Phosphatase antibody
Anti-CDKN3 antibody
Anti-CDI1 antibody
Anti-CIP2 antibody
Anti-KAP antibody

Information sourced from Uniprot.org

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