• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g CDKN2A/p16INK4a antibody (STJ112923). Western blot was performed from the immunoprecipitate using CDKN2A/p16INK4a antibody (STJ112923) at a dilution of 1:1000.
  • Western blot analysis of lysates from wild type (WT) and CDKN2A/p16INK4a knockout (KO) 293T cells, using [KO Validated] CDKN2A/p16INK4a Rabbit polyclonal antibody (STJ112923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of lysates from HeLa cells, using [KO Validated] CDKN2A/p16INK4a Rabbit polyclonal antibody (STJ112923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDKN2A antibody (1-156) (STJ112923)

SKU:
STJ112923

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Current Stock:
Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDKN2A
CDKN2A
Gene ID: 1029
1029
Uniprot ID: ARF_HUMAN
CDN2A_HUMAN
Immunogen Region: 1-156
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-156 of human CDKN2A/p16INK4a (NP_000068.1).
Immunogen Sequence: MEPAAGSSMEPSADWLATAA ARGRVEEVRALLEAGALPNA PNSYGRRPIQVMMMGSARVA ELLLLHGAEPNCADPATLTR PVHDAAREGFLDTLVVLHRA GARLDVRDAWGRLPVDLAEE LGHRDVARYLRAAAGGTRGS NHARIDAAEGPSDIPD
Tissue Specificity Q8N726 - NA
P42771 - Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific.
Alternative Antibody Names Anti-Tumor Suppressor Arf antibody
Anti-Alternative Reading Frame antibody
Anti-Arf antibody
Anti-Cyclin-Dependent Kinase Inhibitor 2a antibody
Anti-P14arf antibody
Anti-CDKN2A antibody
Anti-CDKN2 antibody
Anti-MLM antibody
Anti-Cyclin-Dependent Kinase Inhibitor 2a antibody
Anti-Cyclin-Dependent Kinase 4 Inhibitor A antibody
Anti-Cdk4i antibody
Anti-Multiple Tumor Suppressor 1 antibody
Anti-Mts-1 antibody
Anti-P16-Ink4a antibody
Anti-P16-Ink4 antibody
Anti-P16ink4a antibody
Anti-CDKN2A antibody
Anti-CDKN2 antibody
Anti-MTS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance