![Western blot analysis of lysates from wild type (WT) and CDKN2A/p16INK4a knockout (KO) 293T cells, using [KO Validated] CDKN2A/p16INK4a Rabbit polyclonal antibody (STJ112923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/92708/373160/STJ112923_2__03745.1713139331.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CDKN2A/p16INK4a knockout (KO) 293T cells, using [KO Validated] CDKN2A/p16INK4a Rabbit polyclonal antibody (STJ112923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western blot analysis of lysates from HeLa cells, using [KO Validated] CDKN2A/p16INK4a Rabbit polyclonal antibody (STJ112923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/92708/373161/STJ112923_3__24011.1713139332.jpg?c=1 "Western blot analysis of lysates from HeLa cells, using [KO Validated] CDKN2A/p16INK4a Rabbit polyclonal antibody (STJ112923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
| Host: | Rabbit |
| Applications: | WB/IP/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDKN2ACDKN2A |
| Gene ID: | 10291029 |
| Uniprot ID: | ARF_HUMANCDN2A_HUMAN |
| Immunogen Region: | 1-156 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-156 of human CDKN2A/p16INK4a (NP_000068.1). |
| Immunogen Sequence: | MEPAAGSSMEPSADWLATAA ARGRVEEVRALLEAGALPNA PNSYGRRPIQVMMMGSARVA ELLLLHGAEPNCADPATLTR PVHDAAREGFLDTLVVLHRA GARLDVRDAWGRLPVDLAEE LGHRDVARYLRAAAGGTRGS NHARIDAAEGPSDIPD |
| Tissue Specificity | Q8N726 - NAP42771 - Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific. |
| Alternative Antibody Names | Anti-Tumor Suppressor Arf antibodyAnti-Alternative Reading Frame antibodyAnti-Arf antibodyAnti-Cyclin-Dependent Kinase Inhibitor 2a antibodyAnti-P14arf antibodyAnti-CDKN2A antibodyAnti-CDKN2 antibodyAnti-MLM antibodyAnti-Cyclin-Dependent Kinase Inhibitor 2a antibodyAnti-Cyclin-Dependent Kinase 4 Inhibitor A antibodyAnti-Cdk4i antibodyAnti-Multiple Tumor Suppressor 1 antibodyAnti-Mts-1 antibodyAnti-P16-Ink4a antibodyAnti-P16-Ink4 antibodyAnti-P16ink4a antibodyAnti-CDKN2A antibodyAnti-CDKN2 antibodyAnti-MTS1 antibody |
Information sourced from Uniprot.org